Journal: The Journal of reproduction and development

Article Title: Expression pattern of progranulin in the human placenta and its effect on cell proliferation in the choriocarcinoma cell line BeWo.

doi: 10.1262/jrd.10-073k

Figure Lengend Snippet: Fig. 1. A: Immunoblot detection of progranulin protein in human placenta lysates at different times of gestation. SDS-PAGE (10% acrylamide) was performed with 10 μg protein per slot. Recombinant human progranulin (1 μg) was used as a positive control (left lane). The two bands represent the glycosylated (88 kDa) and deglycosylated (68 kDa) protein. B: Quantified staining intensity of Fig. 1A. Values represent the amount of both bands per lane (i.e., the glycosylated and deglycosylated forms). C: Semiquantitative measurement of the staining intensity of the villous trophoblast cells in representative immunostained pictures. Values are shown as means + SD. The levels of the 8th week and 39th week specimens are significantly different (Mann-Whitney U test, n=6).

Article Snippet: For quantification of progranulin levels, we used the monoclonal Quantikine® human progranulin immunoassay (R&D Systems) following the instructions provided in the manual.

Techniques: Western Blot, SDS Page, Recombinant, Positive Control, Staining, MANN-WHITNEY

Journal: The Journal of reproduction and development

Article Title: Expression pattern of progranulin in the human placenta and its effect on cell proliferation in the choriocarcinoma cell line BeWo.

doi: 10.1262/jrd.10-073k

Figure Lengend Snippet: Fig. 2. Representative photographs of immunostaining of frozen sections of first and third trimester placentae. A: progranulin, 7th week p.m. B: progranulin, 8th week p.m. C: progranulin, 14th week p.m. D: progranulin, 39th week p.m. E: progranulin (without hemalaun), 12th week p.m. F: negative control without primary antibody (without hemalaun), 8th week p.m. Intensity of staining was strongest during the first trimester and clearly diminished at term (see Fig. 1C). Progranulin was mainly localized to the villous trophoblast cells (arrows), whereas expression in the villous stroma (asterisks) was only weak. Scale bar=100 μm.

Article Snippet: For quantification of progranulin levels, we used the monoclonal Quantikine® human progranulin immunoassay (R&D Systems) following the instructions provided in the manual.

Techniques: Immunostaining, Negative Control, Staining, Expressing

Journal: The Journal of reproduction and development

Article Title: Expression pattern of progranulin in the human placenta and its effect on cell proliferation in the choriocarcinoma cell line BeWo.

doi: 10.1262/jrd.10-073k

Figure Lengend Snippet: Fig. 3. Differential expression of progranulin (upper lane, green) using double immunofluorescence staining. Corresponding frozen sections stained for cytokeratin 7 (lower lane, red). A–C: 12th week p.m. D: 39th week p.m. A: Strong progranulin expression of the glandular endometrial epithelium (arrow) with only weak staining of surrounding decidual stroma (asterisks). B: Maternofetal interface. Placental villi with strong progranulin expression of the villous trophoblast cells (VT, arrow). The cytokeratin 7-positive cells in the decidua on the left side of the photographs correspond to extravillous trophoblast cells (EVT, arrowhead). In contrast to VT, the EVT did not obviously express progranulin. C: Placental villus with an outgrowing cell island of EVT cells (arrowhead). Progranulin expression of EVT is weak like that in B. D: Maternofetal interface (39th week). Cytokeratin 7-positive VT (arrow) and EVT (arrowhead). The progranulin expression in the VT is much weaker compared with first trimester specimens. Scale bar=100 μm.

Article Snippet: For quantification of progranulin levels, we used the monoclonal Quantikine® human progranulin immunoassay (R&D Systems) following the instructions provided in the manual.

Techniques: Quantitative Proteomics, Double Immunofluorescence Staining, Staining, Expressing

Journal: The Journal of reproduction and development

Article Title: Expression pattern of progranulin in the human placenta and its effect on cell proliferation in the choriocarcinoma cell line BeWo.

doi: 10.1262/jrd.10-073k

Figure Lengend Snippet: Fig. 4. Immunostaining of progranulin. A: Isolated trophoblast cells after 72 h of culture (9th week of gestation). About one third of the cells show strong protein expression. B: Negative control without primary antibody. C: Jeg-3 cells, 6th passage. D: BeWo cells, 5th passage. Scale bar=100 μm.

Article Snippet: For quantification of progranulin levels, we used the monoclonal Quantikine® human progranulin immunoassay (R&D Systems) following the instructions provided in the manual.

Techniques: Immunostaining, Isolation, Expressing, Negative Control

Journal: The Journal of reproduction and development

Article Title: Expression pattern of progranulin in the human placenta and its effect on cell proliferation in the choriocarcinoma cell line BeWo.

doi: 10.1262/jrd.10-073k

Figure Lengend Snippet: Fig. 5. A–C: Studies were performed on isolated first trimester trophoblast cells. A: Secretion of progranulin in culture medium (n=4). Progranulin levels significantly increased over time (ANOVA, P<0.05). Mean ± SD. Asterisks denote significance compared with the start point at 0 h (post hoc analysis, P<0.05). B and C: No influence of migration or invasion by addition of progranulin (n=3). D: Control of proliferation revealed significant stimulation of DNA synthesis in BeWo cells, but not in Jeg-3 cells (n=3). All values are shown as means + SD. Asterisks denote significance compared with the control (post hoc analysis, P<0.05).

Article Snippet: For quantification of progranulin levels, we used the monoclonal Quantikine® human progranulin immunoassay (R&D Systems) following the instructions provided in the manual.

Techniques: Isolation, Migration, Control, DNA Synthesis